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guaA [2019-07-23 11:45:27]
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guaA [2019-07-23 11:45:27]

GMP synthetase (glutamine-hydrolysing)
Locus
BSU_06360
Isoelectric point
4.75
Molecular weight
57.78 kDa
Protein length
513 aa Sequence Blast
Gene length
1542 bp Sequence Blast
Function
biosynthesis of GMP
Product
GMP synthetase (glutamine-hydrolysing)
Essential
no
E.C.
6.3.5.2
Synonyms
guaB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

    • Gene

    Coordinates
    692,740 694,281

    The protein

    Catalyzed reaction/ biological activity

  • ATP xanthosine 5'-phosphate L-glutamine H2O = AMP diphosphate GMP L-glutamate (according to Swiss-Prot)

    • Effectors of protein activity

  • the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed

    • Structure

  • 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    guaA
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • MGNA-A918 (yebB::erm), available at the NBRP B. subtilis, Japan
  • BKE06360 (guaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA
  • BKK06360 (guaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA

    • References

    Reviews

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    Original publications

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